FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602852
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA151650.32946964186552163No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG146550.3183895549976406No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA122140.26535721765548836No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG103750.22540372794954086No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC96040.20865324368456775No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG87960.19109890998016013No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC79620.17297970910209584No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT72310.157098251258133No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG72060.15655510974500159No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT70180.15247068556625326No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG68830.1495377213953436No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC67240.1460833413718277No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA66260.14395422664035254No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT59980.1303105118304912No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG59570.12941975974895564No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT56760.12331484914135844No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA52190.11338622228131601No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA50680.11010564754200221No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA49870.10834586903945641No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA48120.10454387844753643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16600.076.907571
GCGAAAG23450.055.046332
GCAGGTA31050.054.1848142
ACTGATC33400.052.9652188
CGAAAGC24750.052.149913
CAGGTAC33500.050.6500363
GGTACTG34000.050.54285
CAATTCG48350.050.532029
GTACTGA42900.049.353216
AGTAGAA50650.041.9616621
GTAGAAA53800.040.178941
GCTAAAC26650.037.56181
TACTGAT47450.037.2821587
GGTCAAA47050.035.9220241
TATATTC72750.035.682793
AAGGTCG37200.035.080269
ATATTCA74400.033.525514
GGATAGC34800.032.9205975
GAGCGAA8250.032.5321121
GGTCGTT29950.032.4380957
Produced by FastQC (version 0.11.5)