FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700591
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA51350.13876161942781573No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA45090.12184540253164969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA9000.067.703291
CAATTCG8050.048.6329089
GCGAAAG14050.044.387442
CGAAAGC14750.042.77143
AGTACTG23700.028.1484175
GCTATTG25850.027.7670948
GTAGAAA26050.026.7323111
TATACGT6150.025.9395284
CTATTGA28900.025.5879029
GTTATCG5300.024.6223189
TAGAGCT32500.024.319674
TTAGAGC32750.024.1346723
GTATTAC17100.023.7555391
TAGTACT30300.023.4529744
CTAGTAC15450.022.99823
TAGGTAC14850.022.945827145
GGTCGTT5400.022.825327
GAGCTAT31950.022.4687466
AACCGTA6850.022.2274887
CAATAGG34350.021.962391
Produced by FastQC (version 0.11.5)