FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094680
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA92370.22558539373040137No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG74660.18233415065401937No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC74090.1809421004816005No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA71570.17478777340353824No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA58070.1418181640567761No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC49800.1216212255902781No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC47410.11578438363925875No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA46990.1147586624595817No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT45690.11158381118915275No Hit
TTCTAAACACCTGTGAACTCAACCTGAGATCTGAGAAAAGAACTATTCTA42800.10452587259566072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA10700.052.1989631
CGAAAGC11750.047.523343
GCGAAAG15450.036.142352
GCTATTG33500.034.6285138
CTATTGA33400.034.513019
GCATTAA25400.033.697931
GGAGCTA33500.031.5996747
TAGAGCT38350.030.6328814
CATTAAG31650.027.953762
GAGCTAT42100.027.732056
AGCTATC36050.027.5516389
CAATAGG53150.026.476061
AGAGCTA45550.025.3131945
ATAGGAG55700.024.9977053
TTAGAGC46950.024.7137413
TAGGAGA62400.023.9398174
ACCGTGC22800.023.8498158
GTCTAGT16850.023.6765121
AGGAGCT70900.023.5244676
TACCGAT1550.001808555723.3890637
Produced by FastQC (version 0.11.5)