FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963039
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC153460.3872280843060086No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG124130.31321922393395574No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG109290.27577321343544686No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTTCTCACATA82630.20850160697384001No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC55750.140674870976541No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC53620.13530020774461216No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT45390.11453331647758198No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTA42570.10741756515643677No Hit
GTGGTAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCA42270.10666057033503834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA34200.072.941452
CAGGTAC34500.072.095163
ACTGATC40400.062.8109788
GGTACTG40500.061.5903365
GTACTGA44350.059.18676
GACAGTA37800.055.625197
TACTGAT53750.048.56127
AGCGAAA8250.047.4802971
AGGTACT60700.043.1248634
TAGACCG1053.8226917E-641.435365
CAATTCG5350.039.298569
ACAGTAT61750.039.0981458
CTGATCC69400.038.3388449
CAGTATG55650.038.171129
AGCTATC16150.038.1574069
GTAGAAA29000.038.0091062
TAGACGG1001.3908524E-436.255945
GCGAAAG11500.035.3128052
GGATAGC49250.034.3051155
CGAAAGC12550.032.3575333
Produced by FastQC (version 0.11.5)