FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4790936
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT90670.18925320647155378No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC83330.17393260941077066No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC74130.15472968121469374No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG66290.13836544675194992No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC58020.1211036841235199No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG57030.11903728206763772No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC56700.11834848138234366No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG53910.11252498467940295No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA53710.11210752971861865No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA53560.11179443849803045No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT52250.10906010850489341No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA48820.10190075592744299No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA10000.047.1514631
CGAAAGC11650.042.3263323
AGCTATC27800.041.46539
TACGCGA700.001055752741.4388542
GCATTAA36350.040.1119231
CCCTTAT28700.039.177041
GCGAAAG12250.039.070922
CATTAAG40650.034.2520452
GTAGAAA34550.032.1136132
ATGCCGG6450.031.472467145
TTATACT38100.031.4031374
GAGCTAT36550.031.3412138
CCTTATA38750.028.8200552
CTATACG3102.7284841E-1128.0802781
CAATTCG7400.027.4320819
TATACCC14700.026.6373185
GTATACC14550.025.9151924
AGGAGCT64100.025.56616
TAAGATG62850.025.4977025
GCTATTG28450.024.9741028
Produced by FastQC (version 0.11.5)