FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362227
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT63820.1898146674808096No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC63680.1893982767968968No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC35780.10641756193142224No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT35240.10481148357918725No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC34780.1034433427606167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8500.055.4708751
GCGAAAG9950.048.104762
CGAAAGC10050.046.9024053
TATACTG25350.036.616755
TCGTAAT950.004721992630.5353972
TTAGGTA14450.030.111424
TAGTACT15700.029.0997144
CCTTATA19000.027.1001642
GCTATAT7850.025.8737241
GTAGAAA19450.025.7274482
TTATACT23050.024.2252144
ATTCGAC1500.00149035124.1796111
ATTAGGT19250.023.7332733
CCGACTA2502.2768763E-623.2058664
CCCTTAT21200.022.9250091
TAGTACC7800.022.3133354
ATATACT29700.022.2194214
ATACTGG22750.021.9945726
GATTAGG18700.021.717692
GTACACC13400.021.6472643
Produced by FastQC (version 0.11.5)