FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S6_R1_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S6_R1_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4667859
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA528311.1318036813022843No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA505741.0834517495065725No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG494781.0599720342880965No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA450320.9647249413489138No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG367380.7870417679711404No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT366230.7845781117210268No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG337310.7226225128051211No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC311290.6668796122590679No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT284320.6091015174194422No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG269250.5768169089940377No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG243280.5211811239371198No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC243100.5207955081762323No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG232420.4979156396969146No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC230790.4944236747511011No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT227990.4884252073595196No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA222300.47623546469591305No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC218150.46734487909767625No Hit
GGATACACCATGGACACAGTAAATGCTGTGGTAATGCCAGCCCATGGTCC211140.45232728752089557No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA206900.4432438940422151No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT198960.42623395436751627No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT195930.419742755725912No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT191910.4111306703994272No Hit
GTTCATTGATGCTTAATGCTGGGCCATGTCTTCTTTGCCCAAAATGAGAA175420.3758039820825779No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC173030.3706838617019066No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG171800.36804882066917616No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG170420.3650924331690396No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG167260.3583227342556834No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA160590.3440335280050233No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA159540.3417841027331803No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG155890.3339646720262973No Hit
GCTTAATGCTGGGCCATGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG148370.3178545024603357No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT144960.31054922610130253No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA143580.307592838601166No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC142030.30427225843796907No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG135200.28964028262207575No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC134120.2873265880567515No Hit
GCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAA133400.285784125013202No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG133210.28537708615448754No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT130770.2801498502846808No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA129240.2768721163171381No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA125360.2685599543602324No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT125000.2677887228384576No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC123750.26511083561007304No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC122980.26346125707738816No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT122410.2622401405012448No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG120180.2574627896858067No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT115510.24745820300056195No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA115360.24713685653315579No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA114320.24490885435913978No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC111000.23779638588055035No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC109530.23464719049997013No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC107630.23057680191282556No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC106320.22777037609747852No Hit
GGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGAC104160.22314298696682996No Hit
ATGCTGGGCCATGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG103440.22160052392328047No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT103030.2207221769123703No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC102690.21999379158624974No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT102240.21902975218403126No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA101800.21808713587963988No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG101160.216716057618707No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA100270.2148094019120972No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG96160.2060045087051687No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC94200.20180558153106168No Hit
CCCAATTACAGCAGACAAGAGAATAGGATGCAATTTTCCTCATTGACTGT93260.19979181033531646No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA92950.1991276943026771No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG91900.19687826903083405No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC88630.18987291604137999No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA86860.18608102772598745No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC86820.18599533533467913No Hit
GTAAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATA85690.18357452528021947No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC85330.18280329375844473No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC84820.18171071576926381No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG84720.18149648479099306No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC83510.17890428995391677No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG82580.17691194185599865No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA81720.17506955544287006No Hit
GCCATGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC81510.17461967038850146No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT79220.1697137809861009No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA78450.168064202453416No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA77700.16645747011638526No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA75860.16251562011620316No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA75310.16133734973571395No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC74040.15861661631167523No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA73790.1580810388659983No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA73630.1577382693007651No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA73380.15720269185508817No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA73360.15715984565943403No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG73190.1567956529963737No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA73100.15660284511593No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT71270.152682418213575No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG71090.15229680245268762No Hit
TATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGA69990.1499402616917092No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA69880.14970460761561136No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA69250.14835495245250555No Hit
GGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA69090.1480121828872723No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT69050.14792649049596399No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG68690.14715525897418924No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG68480.1467053739198206No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG68120.14593414239804586No Hit
GATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTAC67350.14428456386536098No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAGGATGCAATTTTCCTCATT66020.14143529185435977No Hit
AGAGAATAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAG65660.14066406033258502No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA65270.13982855951732903No Hit
GGATACAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC64510.13820040408247122No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA64340.1378362114194109No Hit
CAGTAAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAA64050.1372149415824257No Hit
GAATAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT63860.13680790272371124No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT62550.1340014769083642No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT62050.13293032201701038No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA61710.13220193669088975No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT60710.1300596269081821No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT60580.1297811266364301No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA60530.1296740111472947No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT60010.12856001006028675No Hit
GTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGC59930.12838862527767012No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT57920.12408258261442773No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT57910.12406115951660066No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA56750.12157608016865977No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG56450.12093338723384746No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG56100.12018357880989979No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATGTCTTCTT55320.11851257717938782No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA55170.11819123071198166No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA55020.1178698842445755No Hit
ATCCAATGATCGTCGAGCTTGCGAAAAAGGCAATGAAAGAATATGGGGAA54250.11622030571189061No Hit
AGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTC54030.11574899755969492No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT53630.11489207364661187No Hit
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT53570.11476353505964941No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC53540.11469926576616818No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA53530.1146778426683411No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT52640.11277118696173129No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA52570.11262122527694175No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG52160.1117428782660316No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG52100.11161433967906914No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA51960.11131441630949006No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA51100.10947202989636147No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA50900.10904356793981994No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC50800.10882933696154919No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATT50230.10760822038540581No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG49240.10548733370052522No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA49040.1050588717439837No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAG48300.10347356250478004No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA48030.10289513886344896No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA48030.10289513886344896No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA48000.10283086956996773No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATGTCTTCTTTGCCCAAAAT47810.10242383071125327No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG47660.10210248424384713No Hit
GCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGG47490.10173829158078682No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT47220.10115986793945576No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG46920.10051717500464345No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA46890.10045290571116222No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG46720.10008871304810192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA10500.0117.444861
GCAGGTA41650.0116.8317642
AGTAGAA67400.0112.037971
CAGGTAC43900.0110.8354953
AGCGAGC203.867151E-4108.74861145
TACGGAG553.6379788E-12105.470154
CAGTACG1250.0104.456851
GGTCAAA53600.0104.07251
GGTACTG47050.0103.4106145
TACCGAT501.9463187E-10101.5269852
CCGCACG2500.0101.4987145
ACTGATC52150.093.563658
GTACTGA52750.093.336086
AGCAGGT54500.088.777671
GTCAAAT63300.087.9854662
TACTGAT55500.087.396397
GCGTCAG259.368639E-487.047381
CGAAAGC15200.080.1468663
TGCGGGC1000.079.748985145
AGGTACT65950.077.2934654
Produced by FastQC (version 0.11.5)