FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244427
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC62000.276239770774456No Hit
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT53060.23640777802084897No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT49210.21925417935178998No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT41710.1858380780484284No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC37010.16489732123165513No Hit
GATAACATACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAATGAATA36530.16275869074823995No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG34470.15358040159025No Hit
CCATAATAAATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCC31840.1418624887332045No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC31430.14003574186195408No Hit
GTATTATCTCACATGCAAAAAATATCTCAGTAAGAAAAAATGCAACCCAC30420.1355357068864347No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT30400.13544659728295907No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT30090.1340653984290868No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC29020.12929803464314055No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG27960.12457522565893211No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG27790.12381779402938925No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA27070.12060984830426652No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA23180.1032780304282563No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA22760.10140672875526804No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4150.064.673951
TATAGCG502.0097753E-458.0058985
GTCGTAG902.0421794E-856.4197651
TATACTG15850.052.1504175
GCGAAAG6300.044.889522
GTAGAAA17250.044.15461
AGATGTA18750.042.5357637
ACCGTAC1201.9664367E-742.3148231
TAGTACT16900.040.7585834
ATATACT21700.039.4279754
CATTAAG24200.038.9537122
AAGATGT22050.038.802136
TATTATC13950.037.4264952
AATATAC22450.037.1443443
GTATTAT15250.037.102271
AGTACTG18650.036.1564945
TACTGTG39750.035.568077
GAGCGAA2154.838512E-1033.7393951
GTACTGT18200.033.066556
GTAATAT26600.032.9973831
Produced by FastQC (version 0.11.5)