FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_H10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_H10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701976
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGGAAGGCAAGAGAGACAAGGGGGAGAGATGGAGGGAAAGAGC38490.5483093439091934No Hit
AGTATATACTGAGTATTTCGGGTTATCTAATCTTAAGAATGTGATTTAGC33120.4718110020855414No Hit
TTGTAATACAGTCTGAAGTCAGGAAGTGTGATACCTTCAACTTTATTCTT28470.4055694211767924No Hit
ACTCTATACCAACCAAACCACCTATATCAATATTCTGATGACCTTTTTCA16680.23761496119525452No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC15600.22222981982289994No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA15320.2182410794671043No Hit
GATATGTACAGTATATATGGAAGGCAAGAGAGACAAGGGGGAGAGATGGA14840.2114032388571689No Hit
ACACTAAGGGATTCCAAAATTAAAGATTTTAGTGAGTTAGGTTAAGTGAC14130.2012889329549728No Hit
ATATTGGTCTTGGCAATAATTTTTTTGGATATGACACCCAAACCACAAGC13710.19530582242127936No Hit
TCAATATTCTGATGACCTTTTTCATAGAAGTAGCAGAAACAACCCTAAAA13430.1913170820654837No Hit
CTTATATACAGAAAATCCTAAAGACCACCAAAAAACTGTTAGAGCTAGTC12690.18077541112516668No Hit
GTATTACAAAGCTATATTAATCAAAACAGTGTGGTACTGCCATAAAAAAT12210.1739375705152313No Hit
ACCTATATCAATATTCTGATGACCTTTTTCATAGAAGTAGCAGAAACAAC12080.17208565535004045No Hit
ATATATATCTCCTCTTAAAAGAGCCTGAAATCTATTAGTTTCCTATTTAT11310.16111661937160243No Hit
TCTCTAGGCTAACCTGTGAATGCTAATTTACTTTATCTTAGGGATCCTGC10970.1562731489395649No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC10080.14359465280864303No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC9870.14060309754179628No Hit
CCTTAAATACTTTGGATATTAACTCCTTATCTGACAGATGATTGATAAAT9620.13704172222412161No Hit
ATCTTAGGGATCCTGCCTTTCAGATGATTACAGTTACCAAGGAAACAGGC9150.1303463366268932No Hit
GTGTAAAAATAGGCATCCATTTCATTCTTCTGCATGTGATTATGCAGGTT9120.12991897158877228No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG8770.12493304614402771No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA8680.12365095102966482No Hit
GTACCACACTGTTTTGATTAATATAGCTTTGTAATACAGTCTGAAGTCAG8530.12151412583906003No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT8500.12108676080093907No Hit
GTACAGTATATATGGAAGGCAAGAGAGACAAGGGGGAGAGATGGAGGGAA8460.1205169407501111No Hit
CCTTATAACAGTCTCCTCCAGGAATGATTTCTTGAATATATACCAAGGGA8280.11795275052138535No Hit
CACCAATAGCATCAAAAAACAAAAAACTTGGGAATAAATTTAACCAAGAA8280.11795275052138535No Hit
GAATTATTTGATATTGGTCTTGGCAATAATTTTTTTGGATATGACACCCA8200.11681311041972944No Hit
GTCTCATACTCACGACAACCATTTACCAAACTCCGTTATGTTTTTGTATG7680.10940544975896613No Hit
GTAATACAGTCTGAAGTCAGGAAGTGTGATACCTTCAACTTTATTCTTTC7660.10912053973355215No Hit
GTTATAAGGTAAAAATGAAAGTAGAATAACACTTCACAAAAAAACCCAGA7530.10726862456836132No Hit
ATTTGATATTGGTCTTGGCAATAATTTTTTTGGATATGACACCCAAACCA7520.10712616955565431No Hit
AATCAAAACAGTGTGGTACTGCCATAAAAAATAGTCCAAAGAAATAGAAG7470.10641389449211938No Hit
GAGAGAGAGTGTGTGTGTTGGGAGAGGGAGAAAGGGAGAGATTCTCAGGC7460.10627143947941241No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA7390.10527425439046348No Hit
GCCCTATTCACCAATAGCATCAAAAAACAAAAAACTTGGGAATAAATTTA7360.10484688935234253No Hit
GTTTTGATTAATATAGCTTTGTAATACAGTCTGAAGTCAGGAAGTGTGAT7320.10427706930151459No Hit
CTCATTAGGTTAAAGCTCTTTGAAGGTAAAGGCCTATTTTCTAGTTCTTG7290.10384970426339363No Hit
ATACCAACCAAACCACCTATATCAATATTCTGATGACCTTTTTCATAGAA7240.10313742919985869No Hit
CTTACTAGAGAGTTCAGAAGCACAACTTGGGGGTATACCTTTTGCAGAAT7240.10313742919985869No Hit
GGTTTATTTCAGGGCTCTCGCTTCTATTTCTTTGGACTATTTTTTATGGC7210.10271006416173772No Hit
CACTAAGGGATTCCAAAATTAAAGATTTTAGTGAGTTAGGTTAAGTGACT7170.10214024411090979No Hit
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG7120.10142796904737483No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG7120.10142796904737483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAC301.10143446E-7120.83227
TCGCCGT203.8657154E-4108.748985145
AGCGAAA1850.0105.87771
GCGAAAG2450.079.908592
CTATAGG554.3733962E-879.140911
ATACTGA7100.073.530926
ATGGGCG502.303701E-672.509654
CAGGACA2200.065.917874
CGGAGCC554.0605682E-665.908489
TCTCGCG350.00356619362.142277145
ATATCTC3300.061.523345
ACTATTC1200.060.41618
TATACTG9000.058.0077255
ACATCGA1750.057.999468
CATCATT1251.8189894E-1257.999469
GGACATC1800.056.39646
GACATCG1800.056.3883637
TACTGAG9700.055.308767
TCTCCGC1206.002665E-1154.374493145
CTCACGA2950.054.0672879
Produced by FastQC (version 0.11.5)