FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_H08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_H08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615728
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA88461.4366733362783566No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT79151.2854702076241458No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG73381.19175999792116No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA65341.06118285996414No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA60280.979003715926513No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG45580.7402619338409168No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG45470.738475430709664No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC41490.6738364992334277No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC38580.6265753709430137No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT36010.5848361614219265No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG34810.5653470363537146No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC31540.5122391705428371No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT31030.503956292388847No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT31010.5036314736377102No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG29540.4797572954291505No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT29200.47423537665982385No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC27970.4542590234649066No Hit
GGATACACCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGA27810.4516604734558116No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG26550.4311968921341891No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA25400.41251981394381937No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG25020.40634825767221894No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC24880.4040745264142608No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA24410.3964412857625445No Hit
GGATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC24360.3956292388847023No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA23660.384260582594912No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC23120.37549047631421667No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA21630.35129147935452015No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC21460.34853051996985684No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG21390.3473936543408778No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG20510.33310162929085574No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG20390.3311527167840345No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA19880.3228698386300444No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC19350.3142621417249175No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG18650.30289348543512723No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA18560.30143180105501133No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA18440.2994828885481901No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC18380.2985084322947795No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT18380.2985084322947795No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG18240.2962347010368215No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG17240.2799937634799782No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT17180.2790193072265676No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16790.27268534157939867No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA15950.25904295403165034No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA15730.25546994776914483No Hit
GGATACACCATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACC15570.2528713977600499No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA15490.2515721227555024No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCCATGGTGTATCCT15470.25124730400436557No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT15130.24572538523503887No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT15110.245400566483902No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC15030.24410129147935453No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC14500.2354935945742276No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA14290.2320829976872905No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT14270.2317581789361536No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT13750.2233128914065951No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC13660.22185120702647923No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACACATCT13550.22006470389522645No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13520.21957747576852116No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13390.21746615388613155No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG13320.21632928825715253No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC13160.2137307382480576No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA13140.21340591949692073No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA12890.2093456851077099No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT12860.2088584569810046No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC12060.19586570693552996No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11740.19066860691734014No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA11560.18774523815710834No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC11450.18595873502585555No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG11170.18141127250993944No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11090.18011199750539197No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACATTCGAGAAAT11030.1791375412519814No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA10870.17653899124288647No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC10810.17556453498947586No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC10670.1732908037315178No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA10330.16776888496219108No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT10320.16760647558662267No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10310.16744406621105423No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA9960.1617597380661591No Hit
CACCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCG9720.15786191305251668No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA9690.1573746849258114No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA9620.15623781929683236No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA9590.15575059117012707No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACATTCGAGA9580.15558818179455863No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT9400.15266481303432686No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT9370.15217758490762157No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC9340.15169035678091625No Hit
CCCAATTACAGCAGACACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGAT9290.1508783099030741No Hit
CCATGGACATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGT9200.1494166255229582No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA9190.14925421614738976No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG8860.14389470675363147No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA8690.1411337473689681No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG8660.14064651924226282No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG8640.14032170049112597No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT8630.14015929111555753No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC8600.1396720629888522No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT8580.13934724423771536No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA8260.1341501442195255No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT8180.13285086921497805No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAGCGCAAAATGCC8130.13203882233713587No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG8040.13057713795701997No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG7840.12732895044565132No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG7660.12440558168541954No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG7630.12391835355871424No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT7580.12310630668087208No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA7490.12164462230075618No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT7460.12115739417405087No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA7270.11807161603825066No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG7150.11612270353142946No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT7150.11612270353142946No Hit
CCCCAGATGTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC7020.11401138164903984No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA6930.11254969726892394No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG6920.1123872878933555No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT6860.11141283163994492No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA6830.11092560351323962No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA6800.11043837538653432No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT6680.10848946287971312No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT6660.10816464412857626No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA6620.10751500662630252No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT6590.10702777849959721No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATA6580.1068653691240288No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTC6550.1063781409973235No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT6510.10572850349504977No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATATGGTGTATCCTGTTC6500.10556609411948133No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC6480.10524127536834446No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA6470.10507886599277602No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG6440.10459163786607074No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG6390.10377959098822856No Hit
CCATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACCGGTTGGA6270.10183067848140738No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG6180.10036899410129148No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG6160.10004417535015463No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCCC151.2294018E-4145.011123
CCTATAC151.2294018E-4145.011123
GGGTATA100.0070903144.99936145
CCATAGT100.0070903144.999369
TGCCGGA100.0070903144.99936145
AGCGAAA2000.0123.279491
GCAGGTA3900.0115.265252
CAGGTAC4000.0112.383623
AGTAGAA13800.0110.877971
TCCCGAC203.8641636E-4108.758342
GGTACTG4350.0103.341265
GCGAAAG2700.091.303312
CGAAAGC2800.088.042473
ACTGATC5150.087.2811668
TCGCCCA259.3821605E-487.0066764
AGGTACT5500.084.370124
CGAGACC353.436301E-582.85678145
CCGCACG353.436301E-582.85678145
TTAGAAA1050.082.85677145
GGTCAAA8100.080.574821
Produced by FastQC (version 0.11.5)