FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_H06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_H06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022442
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4650.063.954841
GCGAAAG5200.058.568082
CGAAAGC6150.049.520983
TATACTG4150.043.682455
CTAGTAC1851.8189894E-1243.115763
TAGTACT5400.034.91364
GTACTAG1956.9612724E-933.47731
ATCGGAG2451.9717845E-929.591297145
GTCTAGG2752.1282176E-1029.013661
ATATACC2252.8123395E-829.0051463
AGCTTCG1504.39935E-528.99947145
ATATAGG3850.026.3683173
GGGATAC5950.025.58777145
ACTGTGC13900.025.036748
GTCCATA1450.001223099525.0117761
CACGACT1450.001225194425.0044354
CGGGAGT2451.909204E-623.6776684
ATAGTGT3452.9594958E-923.1200456
GTAATAT7300.022.8532281
ATATACT10600.021.8906754
Produced by FastQC (version 0.11.5)