FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889879
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT26140.2937478016674177No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT14240.16002175576679525No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC12840.14428927977848674No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA12600.14159228389477668No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT11650.1309166751884245No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG10240.11507182437162805No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA9700.10900358363328047No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA9390.10551996395015502No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA9070.10192396943854165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACG502.3046778E-672.5071648
AGCGAAA1900.061.0586661
GGTCGAA606.800992E-660.4226381
GCGAAAG2400.048.338112
GTAGAAA5900.041.783791
GTATACG1105.258047E-639.5515863
ATGAACG1502.595516E-838.672666
TTAGGTA3700.037.2355044
CAAGGAC10700.036.594463
CTATGCG3000.036.255624
CGAAAGC3400.034.1229363
TATACTG2800.033.665935
ATTAACG1102.4337189E-432.9596563
TAGTACT3600.032.2272154
AGTAGAA5800.031.253091
GATTAGG4000.030.8155462
GGACAGT13750.030.586566
GGATTAG4350.030.0029641
TATGCGG4000.029.0044985
CGAAGGT1255.1487144E-429.0044984
Produced by FastQC (version 0.11.5)