FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108995
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA45140.4070351985356111No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT28200.25428428441967726No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT23220.209378761851947No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT18380.1657356435331088No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG16520.14896370136925774No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC16510.14887352963719402No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC14960.13489691116731817No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13920.12551905103269176No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG13610.12272372733871659No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG12920.11650187782632022No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA12780.11523947357742821No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG12590.11352621066821762No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG12130.10937831099328671No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11990.10811590674439471No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11700.10550092651454696No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG11350.10234491589231692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC100.0070855217145.037253
AGTAGAA8200.065.461351
TCCGAAC350.003562977562.1588253
CAATTCG3800.059.1439329
GTAGAAA9850.054.4809952
GACAGTA3550.051.0625467
ACAATTC5200.043.2244578
CGTACTG855.3352014E-542.656095
AGCGAAA2450.041.450431
TATGATC3450.039.9359936
CCTTATA19400.039.249772
CCCTTAT20650.038.2889561
GTCTATC951.0274078E-438.16772
TTATACT19950.038.1659744
GATCTTA3750.036.7527161
CCACTAT3950.036.728231
GTCTAAG7850.036.0381161
CGAAAGC2850.035.6231842
ATAGTGG3350.034.635763
CCATACG1051.8539952E-434.5233359
Produced by FastQC (version 0.11.5)