FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456783
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC11090.24278486721265893No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG7430.1626592933624938No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC7360.16112683703202615No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT7010.15346455537968795No Hit
CTACTAACCTGTTTTTGAACTCAGAAATAAATCTACTCCCTCTTTTGTGT6890.15083748738460057No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT6790.14864826405536108No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA6370.13945352607255523No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT5900.12916417642512967No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG5900.12916417642512967No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC5580.1221586617715633No Hit
GTACTAGAAATGCTGAAGCAAAAGTCATGACTACTCCTTAAGAATGATGG4940.10814763246443058No Hit
GTTCAGTGTCTATGCTCAACAATGGTAACTGTAACTCTTATTAGTTAATG4680.10245565180840793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1000.079.8010251
GCGAAAG1250.063.819852
TAGACCG350.00356087262.162195
GTAACGG502.0027393E-458.0371131
GCTATAT1153.8198777E-1156.7754331
CGAAAGC1600.049.859263
CATTAAG3600.044.319342
CTATATA1505.329639E-1043.513542
TATTGGG750.001480276238.67872
AAGATGT4200.037.9880076
TAAGATG4200.037.9880075
TATACCA2500.037.711735
GTATATA2002.2191671E-1036.2731931
ATACCAT2401.8189894E-1236.261286
TACCATG2103.765308E-1034.526997
AGATGTA4550.031.8710697
GTAGAAA4100.031.8496341
AGTACTG4650.031.19255
ATATACC2351.2551027E-930.8606624
TAGTACT5200.030.682624
Produced by FastQC (version 0.11.5)