FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584213
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA37500.6418891739827768No Hit
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG25760.4409350699145689No Hit
AAGTAAATCTGACCAAGAAAATTGACTTACTTGCTATGGTTACTTAATAC21470.36750294841093917No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG21160.3621966645726815No Hit
GTACAGGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCA19720.3375481202917429No Hit
GTGTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAAAG19680.3368634385061613No Hit
GTGTATCATTGTACTTAAAATAGCCATTGGGATAGGGGAAAAGGGAAGCA18050.30896265574370996No Hit
GTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCT17210.2945843382464957No Hit
CCTTAGGGAAGGGATTGGCCACCGGCATCCGGGCATCCCGCAGCTCTTCG16620.2844852819091667No Hit
GTGTAACTTCTTCCTTCCCTGAGCCTCCATTTCCATGGGTGTAAAGTGAA16440.28140421387404935No Hit
GGTGTAAAGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGGATTTTGCAT14540.248881829058922No Hit
GTCCAGGGAAAGTGGCCGGAGCGGGGGTAGGGCTGGTGGCCACCATGGTT14490.24802597682694497No Hit
TATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACA13460.23039542084821804No Hit
GAACATGCATTGTGTAACTTCTTCCTTCCCTGAGCCTCCATTTCCATGGG12590.21550359201181762No Hit
CTTAATACACTTGGTACAGTGCCTGGCATACAGCTGATAATCAATAAATG12380.21190901263751405No Hit
CTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAA11890.2035216607641391No Hit
GTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCT11870.20317931987134827No Hit
GGATAAGAGGCCGGCGGGGTGGACAGGCCGCTGTACAGGCTGGGGGCGAA11020.1886298319277387No Hit
GATGAAATAAGGGCTCTGAAAGCTTTACATTGTCTAATATCTGAAGAATG10250.17544970755529232No Hit
GCCATGATGTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACG10150.17373800309133827No Hit
TCCCAAGCCACAGCCCTCATGTCTGGCTGACCAAGCACAAATGTGGCTAC10150.17373800309133827No Hit
GCTTCAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACA9940.1701434237170347No Hit
GTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTGACTGCAAGAATT9490.1624407536292414No Hit
ACACTGGGGATCCCACACTTGGCCGTTATGGGAGGCCCGGCTAGAGTCAG9450.16175607184365975No Hit
TCCCAGGACCGTGGGATCATGACCTGAGCCGAAGGCAGAGGCTTTAACCT9110.15593627666621593No Hit
GTATGGATTTGGAGTTAAATGGGTTGGAATCTGGAAATGGGGATGAAGAA8990.15388223130947104No Hit
CTCCACCACCTCCCGACCCCGATAAGCCATGATGTCCTGGGGCATCAGCA8930.1528552086310986No Hit
CACATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGG8870.15182818595272615No Hit
CTGGTGGACAGAGGACCTTCCGAGAGACTCGGGCGTACGTAGCCACATTT8840.15131467461353992No Hit
CATTAGTCTTTGGAGGCAATTTTTTGCATAGCTTCAATATCCCCATGCAG8820.1509723337207491No Hit
GGGCTATACCTTCGTCATTCCCTCAGGCTGGATTCATGCTGTGTATACTC8740.14960297014958585No Hit
CCTCTAGGCAGCTTGCAGGGTGAGTCCTTTCTCCCACTGGGCACCGGTTC8480.14515253854330526No Hit
CCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCTTCAATAT8480.14515253854330526No Hit
GTATGGGGCACTCCGGGCCGAGGGGGCAGCAGGCTTGGGGCCTTCGGCCA8480.14515253854330526No Hit
GAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACATGTTGTGT8380.1434408340793512No Hit
GCTTTACATTGTCTAATATCTGAAGAATGACAGATTCACGCACATATACA8100.13864806158027979No Hit
GTGTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTGACTGCAAGAA8010.13710752756272115No Hit
CACCGGGAGAGATCTCCATGGCTGGACGGCTGTAGTCACCGGGGATAGGC8000.13693635711632574No Hit
TCCCTAGACTTATTGTGGTGATCGTTTTGCAGTGGATACAGATACCAAAT7980.1365940162235349No Hit
GTCCACCAGTTCTCACTCGGAGGCCCGTGTCTCCTCTCTGTTTTCTCCTG7880.13488231175958085No Hit
GGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCAGCGGC7870.13471114131318543No Hit
ATATTAGACAATGTAAAGCTTTCAGAGCCCTTATTTCATCCATGCAAAAT7680.1314589028316727No Hit
GGTCTAAGCGGGCACATGTTGTGTGGCTGACTCGCTGCTCTTGTCTGGGC7610.13026070970690484No Hit
GCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAAAAGAGTCCCTC7580.12974719836771864No Hit
CCGTAGTGCATGTCACTGCGTGTCTCCTCTGGGGCCGCCTCCTTGGGGGA7570.12957602792132322No Hit
GTATCATTGTACTTAAAATAGCCATTGGGATAGGGGAAAAGGGAAGCAAT7510.1285490052429508No Hit
GTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAAAGCT7330.12546793720783345No Hit
CAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACACGGG7330.12546793720783345No Hit
GTTCAGAAACTCTTCACGGGGAGGCTTGAGGGCAGGCACCATCTCTGCTT7040.12050399426236663No Hit
GCCCCATACTGGCTGCCCACGGGGCCTGACGACGTCTCTAGGAAACTGGC7030.12033282381597123No Hit
CTACAAGACAGCGGAGGCCTTCAAGCTCAAAATGGGTCAGATGCACCTGC6980.11947697158399419No Hit
AATCCATACTGAGGGACTCTTTTTGAAATTCCTTAGTTAGGTGGGAGCGG6960.11913463069120338No Hit
GTGTTGGAGCGCTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAA6890.11793643756643553No Hit
GTGTGAGGCTTTGTGATGGGGATGGTAGGAACCCGCACCTTTGGCCGGGC6840.11708058533445849No Hit
GTCTAAGCGGGCACATGTTGTGTGGCTGACTCGCTGCTCTTGTCTGGGCC6730.11519771042410902No Hit
CATTTGTGCTTGGTCAGCCAGACATGAGGGCTGTGGCTTGGGAAGAGGGA6660.11399951729934117No Hit
CCCTAAGATCGGCCACCTGAACTCCAAGCTCTTCCTGAATGAGGTGGCCA6580.1126301537281779No Hit
ACATGTGCCCGCTTAGACCTATGATCCCATGTTTAAGCCACATTCTGTGT6540.11194547194259628No Hit
CTCCCGCGCTGTGTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTG6510.11143196060341007No Hit
ACATTGAAGATCGAACACGGGTTCCAAATAAGTTCCGTTATCCATTCTAC6500.11126079015701465No Hit
CTTCTTGTGCAGTGGCAGAGACTCCAACTTATCTACAAGACAGCGGAGGC6400.10954908569306059No Hit
CCCCAGGACATCATGGCTTATCGGGGTCGGGAGGTGGTGGAGAGTAACCT6360.10886440390747895No Hit
CCTCTTAAGTAAATCTGACCAAGAAAATTGACTTACTTGCTATGGTTACT6340.10852206301468813No Hit
TTCCATGGGTGTAAAGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGGAT6280.1074950403363157No Hit
AGTGAGAACTGGTGGACAGAGGACCTTCCGAGAGACTCGGGCGTACGTAG6250.10698152899712948No Hit
AAATAAGGGCTCTGAAAGCTTTACATTGTCTAATATCTGAAGAATGACAG6230.10663918810433866No Hit
GCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCTTGAGGGCAGGCACC6210.10629684721154785No Hit
GTATGGGGCAGCGGGCACAGCCAGCGGCGAGGGCCAGTCAGGGCAGCCCC6170.10561216542596621No Hit
GGTCTGGCTGCTGCCTGTCCTCCAAAGAAAGCTGCCTCCAAGCCTCTAGG6170.10561216542596621No Hit
GTTAAAAATATACAACATTGAAGATCGAACACGGGTTCCAAATAAGTTCC6060.10372929051561675No Hit
CTCTTAAGTAAATCTGACCAAGAAAATTGACTTACTTGCTATGGTTACTT6030.1032157791764305No Hit
CTGTGTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAA6010.1028734382836397No Hit
CTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCTTCAAT6000.10270226783724429No Hit
CAGTAATGCCTTTCTTCCCTTGCAGGATTTTGCATGGATGAAATAAGGGC5970.10218875649805807No Hit
ATCGGGACTCCAGTGAGGGCTAACTTGGGATCGCTGTTGGCAAGCTCCTC5960.10201758605166267No Hit
TCATAGGTCTAAGCGGGCACATGTTGTGTGGCTGACTCGCTGCTCTTGTC5940.10167524515887186No Hit
GTACACATAGCGCTCCAACACGTACCAACACATCTCGTAGTAGAATGGAT5940.10167524515887186No Hit
AGCCTGTACAGCGGCCTGTCCACCCCGCCGGCCTCTTATCCCGTCTACAG5850.10013471114131318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2100.0117.4299551
GCGAAAG2800.088.057392
TAACGCG502.2644599E-887.013975
CGAAAGC3000.082.179863
AACGCGA554.386311E-879.103616
CTTATAG702.8048817E-972.517852
TTAACGC608.016832E-872.511644
ATTAGAT406.655574E-572.5116353
GGGCTAT1850.070.569991
GGTACCG1250.069.6111765
TTATAAG1009.094947E-1265.266062
CTTTCGT451.19098135E-464.471351
TTTCGTG451.1919911E-464.460312
CCATAGA451.1924963E-464.4547964
TTATAGT808.069037E-963.4476853
TATAGTA808.069037E-963.4476854
GTTGCTC606.802493E-660.4160239
GTACCGA1450.060.0096366
TACCGAG1450.060.00457
GTAGCAG851.3033059E-859.7103588
Produced by FastQC (version 0.11.5)