FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499513
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG16730.11156955624926226No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT16190.1079683870696686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3500.082.903511
CGTAATA400.00604686754.3854682
CGAAAGC5850.048.3378033
GCGAAAG5900.047.9329532
CAATTCG4500.035.4441769
TATACAG12450.028.5358225
TATATAC20500.027.9416073
CAGCTCG10900.026.6053039
TACGAAA2051.29023265E-524.757564
TATATTC8250.023.7294653
GGATATA4002.7284841E-1123.5756861
TACACCG3554.125468E-922.4661165
GTATTAA10350.022.4280011
TACTGCG2603.2052194E-622.3082667
ACGAAAC1650.002600567921.9709955
TATAATG6300.021.869292
TTAGTAC7350.021.7026883
CAGGACG2704.44503E-621.482754
GTATTAC7800.021.390171
GTAATAC7800.021.380183
Produced by FastQC (version 0.11.5)