FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861937
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT12520.14525423551837316No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC11940.1385252054384485No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC10440.12112254143864343No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC9410.10917271215877726No Hit
CCCCATTTCCCTCCTTCTGAACTCAGTTTGGTGTGTGTCTTTTCTTCACG8620.10000730911887991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.057.3452031
CGAAAGC2750.044.820513
GCGAAAG2800.044.0252572
GCATTAA6350.041.1164251
ATGAACG1601.0095391E-940.7833756
ACCGTCA907.479286E-540.2798738
GTAGAAA7300.036.759151
ATAGTAC3650.033.768883
GTCTAGT3850.032.023941
CATTAAG8350.031.2627372
ATAGGAG5900.028.2641813
TATAGTA4700.027.7706262
TAGTACT5000.027.5514344
TAGTACC2756.7593646E-926.3650054
GTATATG2507.802191E-826.108931
TGAACGT2507.824201E-826.1013587
CAATAGG5300.025.999461
TAGAAAC12500.024.9441912
GACAGTG6450.024.7299677
GTACCTA2651.3751742E-724.623926
Produced by FastQC (version 0.11.5)