FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312363
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT57540.43844576538655844No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC44310.3376352426881892No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC31900.24307299123794254No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG29390.2239471853442988No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA28560.217622715666321No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC27100.20649774490746844No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC25580.19491558356948496No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT24560.18714334372425923No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC23390.17822812743120614No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA23230.17700895255352367No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA22540.17175126089351803No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG20590.1568925670717629No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA20290.1546066141761083No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG19990.15232066128045366No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA19140.14584379474276554No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT18760.14294825440826966No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG18470.14073849994247017No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA18250.1390621344856568No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT18070.13769056274826402No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA17520.13349964910623052No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG17320.13197568050912742No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC15710.11970773330244758No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT15540.11841235999490995No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC15350.11696458982766202No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT15190.11574541494997954No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC14360.10942094527200173No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA13990.106601603367361No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC13490.1027916818746033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.077.9168851
GCATTAA14400.063.9791641
CATTAAG15450.060.0731242
GCGAAAG3650.055.6242142
CGAAAGC4150.048.9206353
TACGCCT450.00963738248.3364077
GATTAGC6100.047.5693781
TAAGATG22100.041.667135
CCATCGG4050.041.172329
ATGAACG2500.040.6041266
GTCTAGT10900.039.9320951
AAGTCCG750.00148364738.666189
TTAAGAT23550.038.4858674
GAACGTC2700.037.5921178
ACCATCG4550.036.647898
TTAGCAC8150.036.4761053
AGATGTA23300.036.4078947
ATAGGAG7900.035.7947773
GACCGTC1051.8533383E-434.5260057
TAGCACA10200.034.1211174
Produced by FastQC (version 0.11.5)