FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171882
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC18270.15590306873900273No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC12720.10854335163437956No Hit
CCCCATTTCCCTCCTTCTGAACTCAGTTTGGTGTGTGTCTTTTCTTCACG12430.1060686997496335No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT11920.10171672574542487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3000.055.625191
GCGAAAG3900.042.7886051
CGAAAGC4300.038.7883952
GCATTAA6700.031.4042261
ACCGTGC6150.027.1145558
AGTAGAA10000.026.84521
GTCTAGT4450.026.0870461
GTAGAAA10650.025.8880231
TAGGACG1701.0300556E-425.5932964
TTGCACG2101.5555528E-524.16629145
CAAGACG3503.5033736E-922.785358145
TATAGGG3854.274625E-1022.6028352
CTCTACG2603.1840736E-622.324491
GCGCACG1650.00260135921.969355145
GCGGTTG3355.4993507E-821.6423768
TACGCCC2053.6620657E-421.223714
CGTGTTC5600.020.7148488
TTAACGT5650.020.5350344
TAGAAAC14550.019.9352153
GACAGTG10950.019.863557
Produced by FastQC (version 0.11.5)