FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658253
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA15000.22787590789559636No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9260.14067539380754815No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC8100.12305299026362206No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC7790.1183435548337797No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG7420.112722615772355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACC3000.065.263924
GTACGAT400.006050322754.3742145
CGAAAGC2000.047.1386343
GAACGTC2100.041.4279678
ATGAACG2150.040.4737476
AGCGAAA2350.040.1362841
GTAGAAA5250.038.695491
GTATTAG2151.2732926E-1137.1206781
TGAACGT2350.037.023557
GTTTAAG5500.036.9366071
GTGTAAG3100.035.10681
GTCCGGA1352.1302114E-532.2462461
AAGTGAT9100.031.8700857
TTAAGAC6300.031.0804183
GCGAAAG3050.030.9105762
GTCTATA1652.370969E-630.7805061
ACCATCG3300.030.7571268
GTGATCA8300.030.571849
CTATATC1703.0028787E-629.8615763
AGTAGAA6400.029.475081
Produced by FastQC (version 0.11.5)