FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157138
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA83530.3872260374626009No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC44290.20531834310090502No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC38830.18000702783039377No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC38580.17884808482350223No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG31950.14811291628073864No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT31630.14662946923191747No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG30720.14241091668683228No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG25980.1204373572761687No Hit
GTGTATTCTTGAAGAACCTTTCAGTCAACTTGGTCCAGCTGTTCTCATAG25620.11876847934624488No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG25590.1186294061854179No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA22710.10527838274602738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC6050.063.5192453
AGCGAAA6250.061.508011
GCGAAAG6850.056.1035352
CGTTATA1451.9270374E-840.0061652
GTAACGC951.0286338E-438.1620063
TTAAGAC22250.034.8689233
ATGAACG11700.032.8446586
ACCATCG15150.032.540998
TAAGACC22650.032.0115364
CCATCGG15500.031.8061929
GGACAGT18100.031.2457376
AGTAGAA26550.031.1441271
ACCGCCG950.004728684730.5260689
AGTGATC46500.029.7793258
GGTACGC1952.606157E-729.7481752
GTAGAAA29200.029.0628931
GTGATCA46800.028.968789
AAGTGAT48300.028.5194347
GTATTAC12100.028.1739881
GTTTAAG21250.027.9892181
Produced by FastQC (version 0.11.5)