FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_F11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_F11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203439
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC58910.4895138016966377No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT50990.4237024061876007No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC40470.3362862596276172No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG35220.2926612815439752No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC31590.26249772526899995No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT30070.2498672554238312No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC28500.2368213095969135No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG28180.2341622633137201No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA25780.2142194161897695No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG25300.21023084676497936No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA24230.2013396607555514No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG23310.19369490269137032No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA22880.19012180924832917No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA20260.16835086780468306No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT20090.16693824946673658No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC18860.1567175403157119No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA18620.15472325560331682No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT17440.14491802243404112No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT15830.13153969582172426No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT15810.13137350542902465No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG13650.1134249430174691No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT13320.11068280153792588No Hit
TGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGA13090.10877161202188064No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC12790.10627875613138679No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT12640.10503232818613989No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG12610.10478304259709051No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC12600.10469994740074072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.065.588241
GCGAAAG3850.062.1578032
CGAAAGC4400.056.031543
GCATTAA14700.055.7655531
TAAGATG16750.049.3469435
TTAAGAT16950.046.1981164
CATTAAG18800.043.973342
TAGTACG750.00148296538.669544
AGATGTA20100.037.8727957
GATTAGC10400.036.272281
CCATCGG1803.2014214E-936.249689
ATTAAGA22700.035.1376883
GTAGAAA14900.034.568221
GTCTAGT12250.034.3476331
ACTGATC4100.033.5972638
TAGCACA11500.032.785044
TTAGCAC11550.032.645833
ACCATCG2257.930794E-1032.2219358
AAGATGT29200.031.5348746
AACAAGG26650.030.2042582
Produced by FastQC (version 0.11.5)