FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_F10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_F10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944008
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT54360.2796284788951486No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA52120.2681058925683434No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT33490.17227295360924438No Hit
GTGCAGGTACAACGGGATCCACATGCCCAGGGCTCCTGTGGCGAAGGACA29580.1521598676548656No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA26720.13744799404117677No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA24830.12772581182793485No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC22640.11646042608878153No Hit
CCATCATAGGTGACCTCTTCACCAAGAACACGCGCACGCTCATGCTGTCC21940.1128596178616549No Hit
ACTTTATACTGTGCAAAGCCAGATCTAAGAGCATTTCATTCCCATGGGGA19930.10252015423804839No Hit
GTATAAAGTCGAATGGGAAAATTCCTGCTAATAATGTAATTTTTTTTTTT19450.10005102859659014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5300.0116.326371
GCGAAAG7900.078.0236742
TTGCGCA808.092684E-963.436916145
GACATCG1102.5465852E-1159.3206837
CTTACGT1206.002665E-1154.382893
TACATCG400.006050730654.37737
ATTGGGT4050.053.71153
GACCGTC1152.4847395E-950.4369167
ACATCGA1301.3096724E-1050.1944278
TACGTTG1351.9099389E-1048.3391045
GAGTTAG1056.908704E-848.3353737
CTCCGAC752.5516198E-548.332886145
GGTGCAT951.9158306E-645.810261
TATAGAC19400.044.4815332
AGCCGAT1053.822852E-641.4335176
CAATATA1053.822852E-641.4335174
GTATATG1252.71104E-740.618431
CAGTCAA6250.040.5996259
GTATTAC11850.040.398081
ATACTTA26350.040.1755756
Produced by FastQC (version 0.11.5)