FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_F08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_F08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661758
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC33730.2029778102467387No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT17160.10326413352606095No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA17070.10272253842015505No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT16720.10061633523052094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2950.071.313191
GCGAAAG3950.051.404122
CGAAAGC4750.044.2732473
GTAGAAA13500.041.913571
GTATACG1803.1959644E-936.2582633
TAGTACT7450.035.0415464
CTATACG5650.030.8146081
CTATGCG3850.030.1367384
AGTACTG7300.028.8079345
GGATTAG7750.026.2089821
GAGCTAT7300.025.8278036
CTAGTAC9600.025.6829363
ATACGCA6900.025.2231393
GCATTAA8700.025.014731
ACCGTAC1450.001222579425.014731
GCTATTG6100.024.9594468
TAGAAAC23150.024.433212
TATAGGG2802.3394023E-723.3088842
GGACAGT13150.023.1611716
AGTAGAA19800.022.7153971
Produced by FastQC (version 0.11.5)