FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_F07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_F07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154926
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC28370.24564344382237477No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA15070.13048455052531505No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT14820.12831990967386656No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC13530.11715036288039234No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA13120.1136003518840168No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA13030.11282108117749536No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG12840.1111759541303945No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT12420.10753935749996105No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC12120.10494178847822286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.091.331171
GCGAAAG3350.073.591082
CGAAAGC4350.061.6742023
GTAGAAA11650.044.8239821
TAGAAAC15800.033.042012
AGTAGAA10700.031.1801321
GTCTAGT2654.5420165E-927.3689441
ACCATCG4650.026.5063178
TTGCACG1658.4201245E-526.363432145
CCATCGG4450.026.0672159
ATGAACG2808.18909E-925.8960196
TATACGT2101.5538688E-524.1696194
TTAGCAC4303.6379788E-1223.6075333
TAGTACT8050.023.4190084
GTATACG1550.001807373123.3899543
TTAGTAC5000.023.2028333
GTATATA7400.022.542391
GAAACAA22500.022.2360484
GCTATTG5550.022.2079968
ATAGGAG7250.022.0026873
Produced by FastQC (version 0.11.5)