FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269980
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT91800.722846029071324No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA45790.3605568591631364No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT41760.3288240759697003No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG38380.3022094836139152No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA36070.28402022079087863No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA35180.27701223641317185No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA34420.27102789020299534No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT33800.26614592355785127No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG33160.26110647411770266No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG32230.2537835241499866No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT28640.22551536244665268No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC26150.2059087544685743No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC24850.19567237279327232No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT24220.19071166475062598No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT22940.18063276587032867No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG22760.17921542071528687No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT21380.16834910785996632No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA20960.16504196916486874No Hit
GTACATGTCTGGCACCTGATAAGAGCTGATAACTGTAGATGGGTTTTGTT20710.16307343422731066No Hit
CCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCC20350.16023874391722703No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG20210.15913636435219453No Hit
GCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAAC19870.15645915683711553No Hit
GCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTC19860.15638041543961323No Hit
GTGCAAGGGACTGCAGGGGGTTTGTGGTCCTCAGAGCTTTGCGGGGGAAG19840.15622293264460857No Hit
CCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAGCAGAGACAGAGG19560.15401817351454353No Hit
CCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGG19360.15244334556449707No Hit
CCCTAAATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCC19100.15039606922943669No Hit
CATCTACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTT19080.15023858643443205No Hit
CTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAG18740.14756137891935306No Hit
GGTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGC18640.14677396494432984No Hit
CATAAAGTGCTTTGTACATGTCTGGCACCTGATAAGAGCTGATAACTGTA17870.14071087733665097No Hit
GATAAGAGCTGATAACTGTAGATGGGTTTTGTTTAATTTTTTAAAAGCCT17720.13952975637411613No Hit
GTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCTGGAAGCCAGAACCAAG17590.13850611820658593No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC17500.13779744562906504No Hit
GTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGAT17370.13677380746153484No Hit
GCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTAAACAAA16700.1314981338288792No Hit
TTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAACAGGTA16620.1308682026488606No Hit
CCCTTTACAGGAAATGTGACAAAAATCTTCAGAATTGCTGAAATCGCTGA16620.1308682026488606No Hit
GGTTTACATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAA16470.12968708168632576No Hit
TTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGAT16450.12952959889132112No Hit
CCTATTGCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTA16180.1274035811587584No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA16170.12732483976125608No Hit
CCCTCAGCCACCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACAT16090.1266949085812375No Hit
GTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCC16080.1266161671837352No Hit
CTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTCATT15800.12441140805367014No Hit
GTCCAAATCCCTCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGG15660.12330902848863763No Hit
CGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGGCA15590.12275783870612136No Hit
ATAAAAAGTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAA15450.12165545914108883No Hit
GGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAACAGGCCCCCAG15190.11960818280602845No Hit
GTCTCAGTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCT15190.11960818280602845No Hit
GGACATGCCTTTGCCAGGAGAACTGTTAGCCACTTCCGCCTCCAGTCTCA14980.11795461345847967No Hit
GCTCTGAGGACCACAAACCCCCTGCAGTCCCTTGCACTTTAGAATGCAGG14930.11756090647096805No Hit
CCTCAGAGCTTTGCGGGGGAAGCACCCCTTGGAAGCTGGGACCAGGGGCT14880.11716719948345643No Hit
GAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGGAAAGCTGGGT14830.11677349249594482No Hit
GACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCAAACGACTTGGT14770.11630104411093087No Hit
ACTTACCACTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCC14670.11551363013590765No Hit
GCAGTGGACTCTGGATCCAGTGCTCCCCCTCAGCCACCCCTTACTCAGAG14620.11511992314839603No Hit
ATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCCCTAATG14600.11496244035339138No Hit
TCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAA14400.11338761240334494No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA14000.11023795650325202No Hit
CCCCCTGCAGTCCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCG13690.10779697318068No Hit
AAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCA13620.10724578339816374No Hit
CAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAA13410.10559221405061496No Hit
TGAATGGACTGAGACTGGAGGCGGAAGTGGCTAACAGTTCTCCTGGCAAA13290.1046473172805871No Hit
TATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTG13280.10456857588308477No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC13180.10378116190806154No Hit
CATTAGGGCACTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACAT13160.1036236791130569No Hit
GGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAA13050.10275752374053135No Hit
ACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGT12970.10212759256051276No Hit
GTACTGGCACTTCCCACCCTCCCCTGCCTGGACTGGACATGCCTTTGCCA12920.10173388557300116No Hit
GAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGGAAAGCTGGGTAC12870.10134017858548953No Hit
ACATGAAGCTCTGGGAAAGCTGGGTACTGGCACTTCCCACCCTCCCCTGC12830.10102521299548024No Hit
GTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGT12810.1008677302004756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTG26850.054.8174557
GGACAGT29350.051.1403356
CAAGGAC28450.050.974043
AGCGAAA2950.049.17521
ACAAGGA29300.049.002252
AACAAGG31800.046.5308761
AGTAGAA8700.044.1870271
AGGACAG36450.041.974575
AAGGACA35900.041.4057584
GTAGAAA11100.041.1676181
CAGTGGG35800.041.1098569
ACAGTGG39250.039.1603248
CCGATTT1303.6997517E-739.038006145
GGGCTGA17500.036.8884281
GCGAAAG3900.035.3270532
TCTACTA2800.033.6656235
TGAGGTG20750.033.1976135
CGAAAGC4300.032.0395553
GTTATCG950.00472804230.5259599
TAGAAAC15000.029.9722162
Produced by FastQC (version 0.11.5)