FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_F02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_F02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249015
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT24040.1924716676741272No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC18240.146035075639604No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA17630.1411512271670076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3700.078.412041
CGCGTAC350.003566867462.14217145
GCGAAAG5200.055.7820132
CGAAAGC5400.053.7160153
GTATACG1157.1231116E-637.8423351
GTAGAAA11450.036.1072041
GGACAGT19800.030.0309316
GACAGTG20000.029.3633527
TACGGAG1255.1536906E-429.0008417
ATTCGAC1306.4676005E-427.8965931
TAGAAAC15000.027.072872
AGTAGAA13950.026.5167621
CCTACAC5650.025.66963
CATACGG1450.001225262925.0047285
GTATACC1450.001225262925.0047284
AGTGATC13350.024.9819978
CAATTCG2051.2907101E-524.7558239
GTGATCA13750.023.7270139
GGTCTAC3152.8534487E-823.0257591
GAACGTC4103.8198777E-1122.988478
Produced by FastQC (version 0.11.5)