FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_E10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_E10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639974
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG12420.19407038410935445No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT10490.1639129089619266No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA8330.13016153781247364No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC350.003563839262.1523
CCTACGC450.00962304548.351781
AGCGAAA2500.046.4177061
CGAAAGC2850.043.251983
TATACAG6300.039.132745
CAGCTCG6450.035.9686629
TACTACG850.002739274634.1226652
TATATAC12700.032.544163
CGATACT900.003624650232.2269634
CCCGATA900.003624650232.2269632
GAACGCG1352.1411382E-532.2219289
TATACTT6350.031.9732075
GTTATAT1204.0473702E-430.219861
GTATTAG1453.4793746E-530.011451
ATGGGCG1000.006068481629.0042695
CCGATAC1000.006068481629.0042693
TAAACTC1000.006068481629.0042693
ATAGTAT1000.006068481629.0042696
ATAGCAT3500.029.0042673
ATATACA9400.028.5414334
Produced by FastQC (version 0.11.5)