FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272433
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20140.15827945361366766No Hit
GTATGAAAAGATCTCCAGAAGGAGTGCTCGATCTTGCCCACGCTCTTGAT17630.1385534641116664No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT15960.12542900097686874No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA15230.11969196020537035No Hit
CTTCTATATCTCAAATAAGTCTGAAAAGAAATTTGCTTTTCTCTCAGGTG14610.1148194050295772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC6100.059.4305343
TATCGCC502.0097346E-458.00423
AGCGAAA6450.052.847821
GCGAAAG7650.046.4449232
TAACCGG951.9203435E-645.7927864
GGTATCG657.32479E-444.630891
ATACTGC3850.041.4315726
GTAGGAT800.002036099836.2526253
TTACGGT1001.3910703E-436.2526256
CGGGTAT5150.035.193954145
GTACATG1251.265412E-534.799789
ATATATG7400.033.313223
TATATAT8500.033.269732
CTTTACG1102.4354862E-432.956934
GTCGCAT1801.2889359E-732.233421
TCGCATC1801.291155E-732.2270852
AGGTTCA6100.030.9014479
TACGAAG950.004726100730.5285243
GTATTAC3251.8189894E-1229.0100781
AGGGTCG1504.397468E-529.0020965
Produced by FastQC (version 0.11.5)