FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_E08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_E08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319328
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG16710.1266553881976279No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC15050.11407322515704964No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA13980.10596303572727933No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT13730.1040681316549031No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA13660.10353755851463776No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC13490.10224902374542191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3850.052.7485661
CGAAAGC5250.038.6764143
TATACGT2450.038.4776234
GCGAAAG5400.037.6020662
GACCGTC1352.1406926E-532.2266777
ACCGTCA1153.1600794E-431.5260968
GTAGAAA10400.031.3828561
GTATACG2451.9681465E-929.5992953
AGCTATC8400.029.3448499
GTATATG2756.7393557E-926.3742791
GTATATA4200.025.9033091
ATAGTAC5200.025.1024783
TAGTACC3801.2732926E-1124.8079414
TAGTACT8500.024.7405914
GAGCTAT10150.023.574698
CCTATAT3203.378591E-822.661963
GTACCTA3655.662514E-921.8581351
GGACCGT2003.099276E-421.7546566
ATAGGAG11700.021.69353
AGTAGAA13150.021.5105821
Produced by FastQC (version 0.11.5)