FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_E07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_E07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341124
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC21930.16351955523874004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4550.070.1612551
GCGAAAG5550.057.493842
CGAAAGC6050.052.734423
TATACGT1458.69215E-735.004744
GTAGAAA12050.033.115531
GCATTAA8250.030.7801071
CTATACG1504.3778928E-529.0212441
CGAAATA1000.006066742429.0082572
GGTGATC6600.026.3642628
ATCGAAG2808.18909E-925.8963663
TAGAAAC16700.025.6210632
GTATACG2001.0647025E-525.3784393
ATGAACG2601.1438533E-725.0995546
GTATATA4650.024.9645121
CATTAAG10600.024.629652
GTATATG3251.4733814E-924.5564371
ACCGTGC5650.024.3811078
AGTAGAA13550.024.0951291
TAAGATG13700.023.8170975
GTTATCG1851.8312885E-423.5131939
Produced by FastQC (version 0.11.5)