FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904086
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9700.10729067809920738No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG9690.10718006915271334No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC9240.10220266656048207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.054.398071
TGGTGCG1056.900518E-848.3377958
CGGTTCG657.320891E-444.634321
CGAAAGC3100.039.7683332
ACCGTGC2750.039.5491078
GCGAAAG3200.038.531971
ACCATCG2252.0008883E-1135.4477168
GTAGAAA7100.031.668361
GCGGTAA1652.3854045E-630.758715145
CCATCGG2451.9717845E-929.5912979
ATTAACG4350.028.3406523
TAACGTG4450.027.702255
TTACGAA1050.007700436327.6246534
GTCGTAA2207.456965E-726.3748231
AACGTGT4700.026.2287276
TTAACGT4750.025.9526374
CGTGTTC4900.025.1553848
TCGTAAT1450.00122450725.0064562
CTATTGA3201.2405508E-924.921429
GTTTACC3801.2732926E-1124.8090363
Produced by FastQC (version 0.11.5)