FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441737
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT124660.5105381947359605No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT52180.21370032890520152No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA44730.18318926239803876No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA39060.15996808829124512No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT39040.1598861793878702No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA37180.15226865137400136No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG36540.1496475664660035No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA35210.14420062439157041No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA34570.14157953948357255No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG32730.13404392037307866No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG31110.12740929919970906No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC28480.11663827840590531No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT28340.11606491608228077No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA27350.11201042536522156No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA27330.11192851646184662No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT26170.10717780006610049No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC25810.10570343980535168No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC25250.10340999051085353No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT24960.10222231141191701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7100.050.057251
GACAGTG46400.048.9077577
GGACAGT48700.046.8995366
GTAGAAA20300.042.5187231
CAAGGAC50250.040.9805833
GCGAAAG9000.040.2847942
CGAAAGC8750.039.7767263
CGAAGTA3400.038.3866775
ACAAGGA57650.035.8475272
AGGACAG66900.035.6579785
AACAAGG63800.034.2196121
ACAGTGG67500.034.0478028
GTATACG2900.032.504333
AAGGACA66550.032.467974
CAGTGGG66950.031.8368669
AGTAGAA18000.031.8334621
TTAGCGA6050.031.1611753
TAGAAAC28900.030.1090492
CCATCGG8050.028.8195849
TTTAGCG6650.028.3508032
Produced by FastQC (version 0.11.5)