FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_D10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_D10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979673
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.063.071341
CAATTCG2850.040.701049
CGAAAGC4250.037.540252
GCGAAAG4450.035.8585361
TAGAAAC9550.025.8189723
TATCCCG1450.00122509225.0046675
GACAGTA5250.024.8592437
CTATACC2051.2883651E-524.7607174
GCTATAC2351.327051E-624.6879773
GGTCTAT2151.8593179E-523.6150841
ATAGGAC5250.023.4829673
TACTGAT6200.023.3890727
TATATAC10500.022.7922943
GTAGAAA11000.022.4155622
AGTAGAA10400.022.317551
TATACAG8150.022.2434145
GTATTAA5950.021.9424671
GTATATA10000.021.7596131
TAGCCTA2003.0990646E-421.7540595
GTGTAGG4358.367351E-1121.6762451
Produced by FastQC (version 0.11.5)