FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_D07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_D07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827340
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC19840.23980467522421253No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA10470.1265501486692291No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC9990.12074842265574008No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT9890.11953972973626321No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA9700.11724321318925715No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT9190.11107887929992506No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG9160.110716271424082No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC8650.10455193753474991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.096.723431
GCGAAAG3300.079.1134342
CGAAAGC3950.066.0947653
GTAGAAA8550.044.1194571
GCATTAA5000.039.1729931
GTATACG1157.1272716E-637.8368573
CCATCGG1803.1977834E-936.2515569
TATACGT1251.2623592E-534.809914
ACCATCG2603.6379788E-1233.4658
GCTATGG2807.2759576E-1231.0802762
ATGAACG1402.7407697E-531.0802766
AGTAGAA9200.030.7517431
ATAGGAG7100.028.5996913
CAATAGG5950.028.0416681
TAGAAAC13900.027.6517582
TATAGGG2903.947207E-1027.5078332
CATTAAG7700.027.312972
ATTAAGA8550.027.142233
TAAGATG8100.026.8594975
TTACGGG1100.00967146426.363174145
Produced by FastQC (version 0.11.5)