FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_D06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_D06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882216
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG175461.9888553370149713No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA166241.884345783798979No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA140831.5963210823653164No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG109541.2416460367982445No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC107531.2188625007934564No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG89251.011657009167823No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG80490.9123615985200904No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC80280.9099812290867543No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT78830.8935453449041958No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT78650.8915050282470506No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT69590.7888090898374094No Hit
CTCGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAG67890.7695394325199271No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA57150.6478005386435974No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC52920.5998530972006856No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC51780.5869310917054327No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC47590.5394370539641086No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG46770.5301422780815582No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG46440.5264016975434587No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT46110.5226611170053592No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC46090.5224344151545653No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA44510.5045249689418464No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA44070.49953752822438047No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA40560.45975135341004925No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT39220.44456232940685725No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG37790.4283531470750927No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC37630.4265395322687414No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG37210.4217787934020693No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC36300.41146385919094647No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG34670.39298765835124283No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT33480.3794988982290051No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA33400.3785920908258295No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA32410.367370349211531No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC30450.3451535678337278No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA30110.3412996363702313No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA28560.3237302429337033No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG27440.31103493928924436No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC25580.2899516671654107No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA25510.28915821068763203No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA25380.2876846486574716No Hit
GCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG24620.27906997832730307No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC24460.2772563635209518No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC24360.27612285426698224No Hit
GAATGAAGTGGATGATGGCAATGAATGGACATGATTCCAGAGAGGAATGA23450.26580792005585935No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC22220.2518657562320339No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT22030.2497120886494917No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT21770.2467649645891709No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA21760.24665161366377394No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA21680.2457448062605983No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA21360.24211757664789574No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGCATTAAG20830.23610997760185712No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC20320.23032908040661243No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG20270.22976232577962766No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG20120.22806206189867334No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA20020.2269285526447038No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG19900.22556834153994032No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT19160.21718037306056567No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT18900.21423324900024482No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC18420.208792404581191No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC18360.2081122990288093No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG18180.20607198237166408No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT17980.20380496386372499No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC17530.19870417222086204No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA17510.1984774703700681No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT17480.19813741759387724No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA17470.19802406666848027No Hit
ATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAG17450.1977973648176864No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA17450.1977973648176864No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17190.19485024075736554No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG17170.19462353890657164No Hit
TAATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC17100.19383008242879296No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG16750.18986280003989953No Hit
CGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAG16390.18578216672560915No Hit
GTTCATTGATGCTTAATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC16310.1848753593224335No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG16270.18442195562084568No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT16240.18408190284465484No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC15770.17875440935099793No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA15730.17830100564941012No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA15450.1751271797382954No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG15450.1751271797382954No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT15350.17399367048432582No Hit
GTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGT14890.1687795279160659No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT14880.16866617699066894No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC14480.16413213997479076No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA13860.15710438260017953No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA13740.15574417149541608No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT13600.15415725853985873No Hit
GTATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG13340.1512101344795379No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA13010.14746955394143837No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC12850.1456559391350871No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA12850.1456559391350871No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCA12760.1446357808065145No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA12140.1376080234319033No Hit
GACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAGCACATCTGGGG12130.13749467250650635No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG12010.1361344614017429No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG11960.1355677067747581No Hit
GAATGAAGTGGATGATGGCAATGAGATAATGGACATGATTCCAGAGAGGA11940.1353410049239642No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA11740.1330739864160251No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA11560.1310336697588799No Hit
AAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCAC11520.13058026605729212No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11520.13058026605729212No Hit
CAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG11450.1297868095795134No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA11400.1292200549525286No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT11280.12785984384776516No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA10900.12355250868268089No Hit
CTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCC10900.12355250868268089No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT10850.12298575405569612No Hit
GTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC10480.11879176981600878No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT10400.11788496241283312No Hit
GCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATATCTC10340.11720485686045141No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT10320.1169781550096575No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG10280.11652475130806968No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10120.1147111365017184No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC10080.11425773280013059No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA10070.11414438187473362No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCGATACCCAATTAC9930.11255746891917626No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA9870.11187736336679453No Hit
CTTAATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA9790.11097055596361888No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA9670.10961034485885543No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA9650.10938364300806151No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA9590.1087035374556798No Hit
ACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGG9530.10802343190329806No Hit
GCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTG9440.10700327357472546No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT9210.1043962022905955No Hit
TTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTAT9200.10428285136519855No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAAT9030.1023558856334503No Hit
ATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACT9030.1023558856334503No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT8980.10178913100646553No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT8840.10020221805090816No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCA100.0070861317145.03142
AGCGAAA2950.0117.984961
AGTAGAA16100.0112.5956341
CCGCACG800.0108.74889145
CAGGTAC7650.0107.108623
GCAGGTA8050.0101.792222
GGTCAAA18850.094.6305161
GGTACTG8850.093.3994755
GTCAAAT19150.092.774662
GCGAAAG3800.091.5987852
TCAAATA20400.087.0853
GGTATGA259.380418E-487.0139161
ACTGATC9600.086.0977558
CGAAAGC4100.084.8916243
TATACGT353.4352932E-582.865694
TACTGAT10150.080.722627
TCTACTA1800.080.5638665
AAATATA22650.079.390095
GTACTGA11250.076.052296
CAAATAT24450.073.248874
Produced by FastQC (version 0.11.5)