FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557719
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA65990.4236322468943372No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC34240.21980857908262016No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG31710.20356688208848966No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC26760.17178964883910383No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG26660.17114768453103543No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG23900.1534294696283476No Hit
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT23660.15188875528898343No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG21510.13808652266551286No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT21400.1373803619266376No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC16770.10765741446307067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4500.062.857021
CGAAAGC5850.048.339133
GCGAAAG6300.046.0431822
GAACGTC10250.035.366698
GTAGAAA11800.035.0343861
TGAACGT10650.034.0383647
GACAGTG20500.031.1226887
ACCATCG12050.030.6853738
GTACCGC2859.094947E-1230.5299786
GTGATCA32750.030.3279919
CCATCGG12050.030.0827339
AGTGATC32900.029.5295738
AACGTCA12400.029.2336259
ATGAACG12450.029.119966
GTTTAAG10500.029.0109311
AGTAGAA13750.029.010931
CTAGTAC3500.029.003483
GCTTATG12350.028.7723272
GGACAGT22500.027.3921786
TGTACCG3503.6379788E-1226.9318055
Produced by FastQC (version 0.11.5)