FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744193
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17670.10130759612038347No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT17660.10125026301561811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.044.0133631
CAGCTCG10750.036.418089
TATACAG12900.035.414595
CAATTCG7100.034.7178089
CGAAAGC5650.033.370963
TATATAC24800.029.5334053
ATACAGC17850.025.1874876
CCTTATA9000.024.979742
TATAATA7850.024.943812
CCTCGCG1801.5210678E-424.166317145
TATATAT11750.024.0710722
TAGTACA9600.023.4164924
AGGTCGT4700.023.143276
TCGGATT6100.022.581642145
GTATTAG4256.184564E-1122.1888711
GTATTAA8300.021.8495621
TACAGCT18350.021.7311767
TAGAAAC21750.021.6719463
TTAGTAC7700.021.6611213
TATACTG12750.021.6124885
Produced by FastQC (version 0.11.5)