FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250295
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG32010.25601957937926645No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT30610.24482222195561848No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA25590.20467169747939487No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT15070.12053155455312548No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA15010.1200516678063977No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA13930.1114137063652978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3100.0114.65981
CGAAAGC5450.066.523723
GAGCGAA1007.166818E-1058.03191
CGCGCTC400.00605148354.3744479
ACCGTCA1505.366019E-1043.4995578
ACAATAG1557.385097E-1042.1030773
CAGCTCG11450.041.7899679
GCGAAAG8750.041.439752
ACGCCCT1252.7164788E-740.6060835
GTATTAC4400.039.5672041
CCGTCAC1752.4301698E-937.285339
ATAACTC1951.70985E-1037.1850553
ATATACT14900.035.5254554
GGACCGT2950.034.4119346
TATTAGG3050.033.2876662
ATAGACC7850.033.2533873
AGCTAGT1102.4366425E-432.954218
TATACTT15500.032.7468385
TATACAG15800.032.5839965
TATACTG7000.031.0760825
Produced by FastQC (version 0.11.5)