FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737288
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC11050.14987359078135004No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT10170.1379379564023828No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG9630.13061381712438017No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC9130.12383220668178514No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC8850.12003450483393194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2350.049.4011651
CGAAAGC2500.046.4118963
ACCGTCA1053.821362E-641.4307638
GCGAAAG2850.040.712192
CTCCGAC907.481184E-540.277176145
GTAGAAA5900.038.1236761
TTGCACG1157.1408886E-637.825523145
ATCTCGA800.00203641836.24946145
GATTAGC2501.8189894E-1234.827821
GACCGTC1102.433806E-432.9585277
GCATTAA5200.030.69761
AACCGTA950.00472370930.537
CATTAAG6150.028.2999382
CTATAGG1358.0430455E-426.8733221
ATATAGC3005.838956E-1026.5901493
TACACCC7950.026.4515274
TATAGCT2207.469189E-726.3686074
TATATAC3751.0913936E-1125.1397783
TATACAG2601.141816E-725.1008835
ACACCGC1751.2536824E-424.861836
Produced by FastQC (version 0.11.5)