FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_C02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_C02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072040
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14360.1339502257378456No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA13530.12620797731427932No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC11260.10503339427633297No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11250.10494011417484421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG4000.052.5618259
AGCGAAA2850.048.3642961
AATATCG450.00963270948.3417325
CGAAAGC3250.042.393963
TTAGCGG700.001055045841.4415662
GCGTATA1053.824718E-641.42804145
GGTCGTT3050.040.41127
GCGAAAG3450.039.9400632
CCTTATA8200.039.799072
GTAGAAA9950.039.359082
TCGTTTT3400.036.2495359
AGTAGAA10900.035.9404371
GGTTCGC2052.910383E-1035.376952
TTCGCAG2103.783498E-1034.5298044
CGCAGTT2750.034.278686
TACACGT850.002739738434.1235735
AGGTCGT3950.033.0437166
CGGTTCG2257.876224E-1032.2428631
CAAGACG2950.031.948742145
CATACGG1153.1590305E-431.5272165
Produced by FastQC (version 0.11.5)