FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459767
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA40390.27668799198776245No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC34170.23407845224614615No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC30920.21181462521073566No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA30680.2101705272142746No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT30150.20653981080542305No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG26760.18331692660541032No Hit
GTTATAATCTCTCCTTTCCCTCACTCCCCTTTTCCCTCCTTCTGAACTCA25670.17584998153814957No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT25390.173931867208945No Hit
GTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCATACGTTTATTTTG24910.17064367121602284No Hit
GGTGTAGGGTTTGTGTAGATGTGCGGTGGTTTGGTCAGAAGTGGGATCAT23520.16112160365318576No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC21840.1496129176779582No Hit
ATATTCAACTGTCTCTCCTTTGACCGATGTTCGAGTTATGTTTTGTTTTC21560.1476948033487536No Hit
CTTTGAAGATGTTATAAAAGAAAAAGTTATAATCTCTCCTTTCCCTCACT21350.14625621760185015No Hit
GAAGTGGGATCATACGTTTATTTTGCACTTCATTTTTCCCACTAAATAAT21000.1438585746903444No Hit
AACTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAA20570.14091289911335167No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC20570.14091289911335167No Hit
AAGTAATATTTTCCCCCTTCTTAAATAAAAAGCATAAATCCTTAGGAATG20380.13961132153281997No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG19390.132829417297418No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC18970.12995224580361112No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG18290.1252939681469714No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT18230.12488294364785613No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG17830.12214278032042099No Hit
ATGTATTAGTTTAGGGAAAATTAATGAAGATAAAAACAATAACAAGCACA17720.12138923540537634No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA17520.12001915374165878No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG17270.1183065516620118No Hit
GGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAAC17060.11686796591510837No Hit
GTATAATACAGTGATTAGACAAGTCTATAAGTTATGCTGTGCTCAGGAAA16920.11590890875050608No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA16740.11467583525316026No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA16730.11460733116997437No Hit
CTAAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATA16470.11282622500714155No Hit
CTATTAACGTGTTCTATTTGACCAAAAAAGAAGTTACAGTTAATAGAGTT16200.11097661476112285No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA16160.11070259842837933No Hit
GCGTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTT15950.10926401268147587No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA15930.10912700451510413No Hit
ATGTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAAC15520.1063183371044831No Hit
GTATAGTCAATGGTATTACCATAGTGGTGTATGGTGACAGACGGTGGCTC15290.10474274319120791No Hit
GTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGCTTCAGGGCT15170.10392069419297738No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT15030.10296163702837509No Hit
CCAGTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTT14860.10179706761421514No Hit
ATGTAAAGACAAGTGGGTAGCGTATAACACAGCTTATCAGAGTTTTTTCT14780.10124903494872811No Hit
GTATTAGTTTAGGGAAAATTAATGAAGATAAAAACAATAACAAGCACATG14720.10083801044961285No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT14620.10015296961775406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC755.638867E-1177.331917
AGCGAAA3550.065.400191
ACCGTCA954.5656634E-1061.051518
GCGAAAG4350.058.3562472
CGAAAGC4350.055.0159453
AGTAGAA8950.048.6391031
GTAGAAA11150.047.501291
GGACCGT1351.0246367E-842.9724776
CGACCAA2450.041.4377445
CAAGACG6300.040.277035145
CCGACCA2550.039.8127334
GTATACG2109.094947E-1237.9871983
TAGAAAC14100.037.5502052
GGACAGT13550.037.4621666
ATGAACG5950.034.1252026
GTCTGAC5450.033.2699432
GTATAAA2506.91216E-1131.9234681
CCCGACC3200.031.7279473
GACAGTG15950.031.3630587
GTGATCA22900.031.3422169
Produced by FastQC (version 0.11.5)