FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030078
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT38240.37123402305456477No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA31530.30609332497150704No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC26160.2539613504996709No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG23560.2287205434928229No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA22190.21542057980075296No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA19400.18833525228186604No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT18970.18416081112304117No Hit
ACTATCCACAGAGTAAAAATTCAATCCACAGAATGGAGAAAAGATTTGCA18310.17775352934437974No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC17980.17454988845504904No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG17010.1651331258409557No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG16890.16396816551756274No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG16770.16280320519416977No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA16100.15629884338855893No Hit
GTTGTAAACTGCACGGGAGTCGGGATCCTGACATGGAGACCTCTCCGTAC15210.14765872099006094No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC13790.13387335716324394No Hit
GTCTATGACCCAGCACTCCTGCTCCCAGATATTCTCCCAAGAGAAGCAAA13540.13144635648950856No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA13160.1277573154654308No Hit
TGGATAGACAGACTGGATTCATGAAAATTAAGAGATTTTATGCTTCAAAA12550.12183543382151643No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT12230.11872887295913515No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA12220.11863179293218572No Hit
CCCTAATGGTTAGTGATATTGGGCATCTTTTCATGTGTTTATAGGCCATT11160.10834131007554768No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG10800.10484642910536873No Hit
ATGTAGGACTGTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACAGA10730.10416686891672282No Hit
GTATTATCTCACATGCAAAAAATATCTCAGTAAGAAAAAATGCAACCCAC10700.10387562883587456No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA10380.10076906797349328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGC352.7620626E-7103.56983145
GTGATCG502.0104258E-457.9991049
AGCGAAA5800.050.0308041
GTACGGG450.00961579548.3631061
GGGTACG450.00962871848.346674
TCGACGG604.9455283E-448.332584145
TATATCC2600.044.6298643
ACTGTAC2300.044.1297538
ATACTAA3000.041.0827037
CCGCTTC2850.040.7011269
GAGCCGC2950.039.3328826
CATACTA3150.039.1377836
ATAAGAG2600.039.0492325
CGAAAGC7550.038.423063
GTCGCGC1701.8299033E-938.3817639
TGTCGCG1701.8299033E-938.3817638
TACCACG1354.9721893E-737.5920147
GTTTAGT16550.036.3819161
GTCTTAG3400.036.272331
TATTATC4000.036.2635232
Produced by FastQC (version 0.11.5)