FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806702
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG9650.11962285949458412No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA8880.11007782303750333No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT8770.10871424640077748No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8760.10859028488834786No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1600.054.3943941
CAATTCG3350.049.7753529
CGAAAGC2100.041.4433443
GGTCGTT2603.6379788E-1233.465177
CAGCTCG3900.033.4610259
GCGAAAG2705.456968E-1232.2337152
AGGTCGT2301.9099389E-1031.5310276
TGTACCG950.00471995330.535315
GTATTAG1703.0024075E-629.8635881
GTATATA8850.029.5020451
GTTACGC1000.00606319929.0103423
CCTTATA5300.028.736662
TATACTT6700.028.1426185
GACAGTA4450.027.6996387
TACTCCG1050.0076965127.6271865
TATATAC10700.027.112473
TAGAAAC9700.026.9168133
GTAGAAA10100.026.5688762
GTATTAA4800.025.686241
TTATACT6700.024.8953914
Produced by FastQC (version 0.11.5)