FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580058
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT10420.17963720869292382No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC8770.15119177737398673No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC7380.12722865644470036No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC6150.10602388037058363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC1800.068.477763
GCGAAAG2000.065.255272
AGCGAAA1900.064.884861
GCATTAA3900.046.486131
GCTAGGC700.001055853641.4319233
ATGAACG1001.3900676E-436.252936
CGAACGC800.002035964336.249805145
CATTAAG4850.035.879192
CCAATAC2206.202754E-1032.957213
GTATACC1102.4337386E-432.957214
GTATAGT2202.255183E-829.6666031
GTAGAAA4050.028.6442932
ATTATAG1804.6925434E-628.1967243
TATACAG1555.4934688E-528.0667865
TGAACGT1358.074784E-426.8540237
GAACGTC1358.0788514E-426.851718
GTCTAGT2808.1581675E-925.8994161
AGATGTA7650.025.5903057
CAGACGT1450.001225860324.9998669
ATTAAGA7350.023.6753833
Produced by FastQC (version 0.11.5)