FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702674
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA25060.3566376441991592No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC23040.3278903161352206No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17510.24919094772255698No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT17260.24563311009088143No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT17190.24463691555401224No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC16170.2301209380167759No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA15300.21773966305854492No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA14490.20621226913191607No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA13970.19881196685803088No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA13640.19411562118421916No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT12460.17732262756271044No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA11860.16878381724668906No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT11800.16792993621508695No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC11570.16465672559394542No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC11320.16109888796226984No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC11140.15853724486746343No Hit
CCATGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGA11030.15697179630952618No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT11020.15682948280425915No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC10370.14757910496190269No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10190.14501746186709627No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT10170.14473283485656221No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG10120.1440212673302271No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10020.14259813227755688No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG9610.13676327856160894No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG9270.13192461938253017No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG9230.13135536536146206No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC9130.12993223030879184No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT9060.1289360357719227No Hit
GAAACAAGGAGGGGAACCAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG8650.12310118205597476No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG8600.12238961452963963No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT8490.12082416597170237No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC8130.11570087978208957No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA8080.11498931225575446No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC7960.11328155019255018No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC7840.11157378812934589No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7750.11029296658194268No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG7720.10986602606614163No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA7390.10516968039232988No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC7360.1047427398765288No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG7230.1028926643080575No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT7170.10203878327645537No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG7150.10175415626592134No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG7150.10175415626592134No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG7110.10118490224485324No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA7100.1010425887395862No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT7050.10033102121325109No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG7050.10033102121325109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2250.077.355161
GCGAAAG2250.077.3386462
AGTAGAA9400.067.89151
GTAGAAA10400.061.3503722
TAGAAAC12600.051.210193
CGAAAGC3550.051.0562133
GGGCGAT901.323031E-648.3332147
GGCGATC951.920096E-645.789368
TATACGT803.7335667E-545.312394
GGTCGTT3050.045.1638227
GATTAGC3100.042.1086541
ACAAGGC6750.041.8887868
TAAGATG6800.041.580785
AGGTCGT3500.039.3570486
GTTCCGA750.0014810338.677581
CAATTCG6400.038.515539
ACCATCG2550.036.9606978
TTAAGAT7150.036.503414
ACCGTGC7950.036.4778988
AGAAACA17700.036.4547124
Produced by FastQC (version 0.11.5)