FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851168
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC13260.15578593180194744No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC12470.14650456784089627No Hit
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC8970.1053846009248468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3100.053.808771
CGAAAGC3750.044.4740753
GTAGAAA8500.041.8084451
GCGAAAG4400.037.9062652
TAGAAAC10950.033.7747342
CGAACGT1102.435985E-432.9542279
GAACGTC2003.251971E-729.0014238
GGACAGT14050.028.901616
GTGTAGG3005.838956E-1026.592451
TAAGACC5200.026.4947974
TATATAC6050.026.3680274
TAGCACG1100.00966294426.3680274
CTTATAT5000.026.105882
GACAGTG15100.025.9284237
CCTTATA5500.025.054041
CATACGG1751.2540919E-424.8612823
CAAGGGT6300.024.170694
TGAACGT2401.5969345E-624.1678527
GTATATA4600.023.649411
CCGAACG1550.00180775323.3882438
Produced by FastQC (version 0.11.5)