FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920372
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT47460.5156610587892722No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT18190.19763747702016143No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC17390.18894533949316147No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA17360.18861938433589895No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT14060.15276431703702414No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA12870.13983476246561172No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA12590.13679251433116174No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT11120.12082071162529934No Hit
GACTAACACAAACACACAATTACAAAGTGGTAAGTGGTTTATTTTCAAAT11010.11962554271533685No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG10580.11495351879457437No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA10190.11071610175016189No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9920.10778250533479941No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG9510.10332778485221193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1900.080.182561
GCGAAAG2450.062.1621172
CGAAAGC2650.057.4675183
GACAGTG17050.048.4821977
GGACAGT17250.047.925296
CAAGGAC16900.044.626793
ACCGTGC2050.042.442958
CCGACCA1053.8170747E-641.4391634
GTAGAAA9600.039.2958221
AGGACAG22950.039.1821255
CGACCAA1157.1295744E-637.835755
CAGTGGG23700.035.484669
GTATACG1854.1709427E-935.279283
ACAGTGG28650.032.9000788
CTATATC2705.456968E-1232.2304574
AAGGACA24150.031.5297974
TACCTAT4850.031.3963667
TATACGT2351.2587407E-930.858954
ACAAGGA24500.030.4890382
ATAACGG1204.0517253E-430.2160573
Produced by FastQC (version 0.11.5)