FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621366
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT11700.18829482140960402No Hit
CAATATACACAGTGCTTAGTACAAGTACTTGTTGGTAGTAACTGCTGGTG9100.14645152776302534No Hit
TCTATAGACAGTGCACATTCAGATGTTGCTTCGGGAATGAGTGATGGCAA8470.13631257584096973No Hit
GTGTAAGCCCAGGTAACGAGAACACAGGTAATAATGAAACACTGATTAAG7620.1226330375334344No Hit
ATGTAGGATTATTCTGAGCTAAAAGCTCTGAAAATACACTTGGCTTCTCA6730.10830975624672094No Hit
GTACATAGCTAGGAAATAACAAAACTGAGTCTTCAGCCTGAATTACTGCA6400.10299887666850134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2100.0120.889761
GCGAAAG2400.0105.7444842
CGAAAGC2800.088.048473
GGGTTCG902.048182E-856.387987
ATATATG4400.054.4003871
TAGACAG2600.044.621855
ATAGACA2850.040.7076534
TATAGAC2850.040.7076533
GTTCGTG1252.7170609E-740.5993469
ATGCCGG907.476328E-540.28037145
GTTAGAA1105.25585E-639.551182
TAGTGCT1105.25585E-639.551184
TATGACA7300.037.7451934
GAGTAAT800.002034575236.2552535
ATCGTGC1209.55908E-636.2494168
CCTTATA2252.0008883E-1135.449582
TATATGA7950.034.6591072
TGATAGG3350.034.6318862
ATGTAGG4400.034.6184271
GTATAGG1051.847833E-434.5399321
Produced by FastQC (version 0.11.5)