FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n01_S5_R2_B01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n01_S5_R2_B01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394902
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17670.12667556573866837No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC16640.11929153445905159No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT15230.10918329746462477No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA14540.10423671340352225No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC14150.10144081806463823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2500.049.32051
GCGAAAG3000.048.3534241
GTAGAAA8600.045.542181
CGAAAGC3300.043.9482042
ATGAACG2750.036.9164926
TAACGAC1707.8127414E-834.124493
CCATCGG3200.031.7182889
CTTAACG1902.0700281E-730.5390071
TAGTACT5700.030.5324364
GGACAGT16100.027.9248546
TAGAAAC15550.025.648232
ACCATCG4101.8189894E-1224.7566248
ATAATAC8950.024.306553
TACCTAT6100.023.771857
ATACCTA6750.023.6343696
GACAGTG20950.023.5335627
TATAAGG4750.022.8993282
TCTCGTT2903.2884418E-722.499672145
TTAACGA2603.2000953E-622.3121662
TATACGT2603.2000953E-622.3121664
Produced by FastQC (version 0.11.5)